Supplemental Figure 4

Supplemental Figure 4. Metyrapone administration does not alter open field behavior. (A) Mice were injected with 50 mg / kg metyrapone 30 minutes before a 10-minute open field test. (B) Representative heatmaps of movement during the 10-minute session. (C) There was no effect of Metyrapone on total distance traveled during the task, and (D) average velocity was also not impacted by metyrapone. Data presented as mean value +/- SEM.

t.test(Distance ~ Condition, data = data, var.equal = T)

## 
##  Two Sample t-test
## 
## data:  Distance by Condition
## t = 1.1024, df = 13, p-value = 0.2903
## alternative hypothesis: true difference in means between group Metyrapone and group Saline is not equal to 0
## 95 percent confidence interval:
##  -395.3512 1219.2112
## sample estimates:
## mean in group Metyrapone     mean in group Saline 
##                  4612.47                  4200.54

t.test(Velocity ~ Condition, data = data, var.equal = T)

## 
##  Two Sample t-test
## 
## data:  Velocity by Condition
## t = 1.1501, df = 13, p-value = 0.2708
## alternative hypothesis: true difference in means between group Metyrapone and group Saline is not equal to 0
## 95 percent confidence interval:
##  -0.646531  2.118472
## sample estimates:
## mean in group Metyrapone     mean in group Saline 
##                 7.888036                 7.152065

Administration of 50 mg / kg metyrapone did not alter distance traveled in the open field (p = 0.29) or the average velocity of metyrapone-injected mice compared to saline-injected controls (p = 0.27; Figure S4).