Supplemental Figure 3 - Sex Differences in Hyperalgesic Priming

• Priming was induced by CFA.

• Expression of priming was elicited by 100ng PGE-2 administration at the site of previous injury.

Published Image

Figure S3. Sex differences in PGE-2-induced expression of Hyperalgesic priming. (A) There is no sex difference in the magnitude of mechanical sensitivity induced by PGE-2 administration in naive mice. (B) Among CFA-primed mice, males exhibit more sensitivity than females 24 hours post PGE-2 administration. Data expressed as mean value +/- SEM, *** indicates p < 0.001.

Statistics

a <- m_Left[m_Left$CFA == "CFA", ]

b <- m_Left %>%
  filter(CFA == "CFA") %>%
  group_by(variable) %>%
  pairwise_t_test(value~Sex)

tt(b)

variable	.y.	group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
Post-CFA Baseline	value	Male	Female	8	8	0.232000	ns	0.232000	ns
3 hours	value	Male	Female	8	8	0.319000	ns	0.319000	ns
24 hours	value	Male	Female	8	8	0.000306	***	0.000306	***

• 24 hours after PGE-2 administration, CFA-primed males exhibit ongoing hypersensitivity, whereas CFA-primed females do not.